vectors prk5 myc tead1 Search Results


tead1 vector  (Addgene inc)
93
Addgene inc tead1 vector
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Tead1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectors+prk5+myc+tead1/pRK5-Myc-TEAD1+(Plasmid+%2333109)/pmc05644195-42-11-23
Average 93 stars, based on 1 article reviews
tead1 vector - by Bioz Stars, 2026-09
93/100 stars
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prk5 myc tead1  (Addgene inc)
94
Addgene inc prk5 myc tead1
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Prk5 Myc Tead1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectors+prk5+myc+tead1/pEGFP-C3-hYAP1+(Plasmid+%2317843)/pmc06701825-188-7-8
Average 94 stars, based on 1 article reviews
prk5 myc tead1 - by Bioz Stars, 2026-09
94/100 stars
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prk5 myc tead1 plasmid  (Addgene inc)
88
Addgene inc prk5 myc tead1 plasmid
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Prk5 Myc Tead1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectors+prk5+myc+tead1/pRK5-Myc-TEAD1+(Plasmid+%2333040)/bio_rxiv__201004-253-21-23
Average 88 stars, based on 1 article reviews
prk5 myc tead1 plasmid - by Bioz Stars, 2026-09
88/100 stars
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prk5 myc tead1 tyr 406ala  (Addgene inc)
85
Addgene inc prk5 myc tead1 tyr 406ala
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Prk5 Myc Tead1 Tyr 406ala, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectors+prk5+myc+tead1/pRK5-Myc-TEAD1-Y406A+(Plasmid+%2333047)/pmc04648367-298-17-20
Average 85 stars, based on 1 article reviews
prk5 myc tead1 tyr 406ala - by Bioz Stars, 2026-09
85/100 stars
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human oc2 dna binding region  (Addgene inc)
92
Addgene inc human oc2 dna binding region
Fig. 4 | <t>DNA</t> binding thermodynamics of <t>OC2</t> base-specific mutants. a–c ITC binding analysis of OC2SQ, OC2N and OC2RR, to PEG10 DNA. The raw heats (differential power, DP) for each injection are shown on top and binding isotherms are shown in the bottom. The data shown is representative of three independent experiments (n = 3; technical replicates). Source data are provided as a Source Data file.
Human Oc2 Dna Binding Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectors+prk5+myc+tead1/pRK5-Myc-TEAD1-V242A+(Plasmid+%2333048)/pm39426953-234-5-20
Average 92 stars, based on 1 article reviews
human oc2 dna binding region - by Bioz Stars, 2026-09
92/100 stars
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pac sgrna cas9puro  (Addgene inc)
90
Addgene inc pac sgrna cas9puro
Fig. 4 | <t>DNA</t> binding thermodynamics of <t>OC2</t> base-specific mutants. a–c ITC binding analysis of OC2SQ, OC2N and OC2RR, to PEG10 DNA. The raw heats (differential power, DP) for each injection are shown on top and binding isotherms are shown in the bottom. The data shown is representative of three independent experiments (n = 3; technical replicates). Source data are provided as a Source Data file.
Pac Sgrna Cas9puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectors+prk5+myc+tead1/pRK5-Myc-TEAD1-I247A+(Plasmid+%2333049)/pm36018884-82-15-18
Average 90 stars, based on 1 article reviews
pac sgrna cas9puro - by Bioz Stars, 2026-09
90/100 stars
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prk5-myc-tead1-y346a (plasmid #33044)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prk5-myc-tead1-l272a (plasmid #33050)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prk5-myc-tead1-e398k (plasmid #33045)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prk5-myc-tead1-d278k (plasmid #33043)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prk5-myc-tead1-h404a (plasmid #33046)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prk5-myc-tead1-e205k (plasmid #33042)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the YAP-TEAD1 interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer

doi: 10.1186/s13046-017-0612-3

Figure Lengend Snippet: G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the YAP-TEAD1 interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells

Article Snippet: DNA plasmids that encode wild type human YAP (hYAP, CMV2-YAP) and TEAD1 vector (pRK5-Myc-TEAD 1) and YAP, COX-2, LATS1 shRNA were obtained from Addgene (USA).

Techniques: Co-Immunoprecipitation Assay, Luciferase, Activity Assay, Control

Fig. 4 | DNA binding thermodynamics of OC2 base-specific mutants. a–c ITC binding analysis of OC2SQ, OC2N and OC2RR, to PEG10 DNA. The raw heats (differential power, DP) for each injection are shown on top and binding isotherms are shown in the bottom. The data shown is representative of three independent experiments (n = 3; technical replicates). Source data are provided as a Source Data file.

Journal: Nature communications

Article Title: The homeodomain regulates stable DNA binding of prostate cancer target ONECUT2.

doi: 10.1038/s41467-024-53159-8

Figure Lengend Snippet: Fig. 4 | DNA binding thermodynamics of OC2 base-specific mutants. a–c ITC binding analysis of OC2SQ, OC2N and OC2RR, to PEG10 DNA. The raw heats (differential power, DP) for each injection are shown on top and binding isotherms are shown in the bottom. The data shown is representative of three independent experiments (n = 3; technical replicates). Source data are provided as a Source Data file.

Article Snippet: Protein expression and purification The human OC2 DNA binding region spanning residues 330–485 (OC2) was cloned into pET-His6-TEV-LIC expression plasmid (Addgene Plasmid #29653).

Techniques: Binding Assay, Injection